ABSTRACT
Objective: To investigate the anti-diarrhoea property of aqueous leaves extract of Psidium guajava (red apple guava leaves) in castor oil-induced diarrhoea in rats. Methods: Five groups of five rats each orally received the following treatment; groups II, III & IV received 100, 200 & 400 mg/kg body weight of the extract respectively and group V received a standard drug for diarrhoea (Laperamide), while group 1 received 1ml of castor oil only without treatment. 1 ml of castor oil was given to rats in all groups, one hour after the treatment with extract and standard drug. The rats in each group were then placed singly in a cage with adsorbent paper on the floor of the cage. The diarrhoea episode was observed for 4 hours and the cumulative frequency of the wet and formed stools were noted at the end of the 4th hour. Percentage inhibition of diarrhoea was calculated using the mean stool frequency and the anti-diarrhoea activity determined in terms of percentage protection. Results: The extract reduced stooling in castor oil induced diarrhoea in rats in group IV which shows no significant difference with the group V treated with standard drug. The rats in group I showed the highest stooling episode which is significantly higher than all other groups. Conclusion: These findings suggest that an aqueous extract of guava leaves may be used as an effective treatment for non specific diarrhoea in medicine. The anti-diarrhoea action may be linked to direct inhibitory effect of the extract on the propulsive movement of the gastrointestinal tract smooth muscles. It equally shows that the treatment is dose dependent.
ABSTRACT
The seeds of Cola nitida were investigated for their anti-diabetic properties. The extracts were obtained by maceration in cold water. Alloxan was used to induce diabetes mellitus (DM) in the rats at 180mg/kg body weight and diabetes was confirmed after 48 hours. The Phytochemical analyses of the plants showed the presence of tannin, flavonoid, terpernoid, cardiac-gylcoside, and alkaloids. Anthraquinone and saponin were observed to be absent. Cola nitida was also observed to cause a reduction in blood glucose level from 599±0.667mg/dl (diabetic control) to 170±0.577mg/dl (extract treated group). The extract showed a significant (P<0.05) decrease of blood glucose level in alloxan induced diabetic rats. Cola nitida aqueous extract showed a higher level of potency when compared to a known anti-diabetic drug (Glanil).